The use of organic waste from agricultural activity in the form of biochar can be a viable alternative both in the process of management of biomass generated, as in the recycling of nutrients contained in these residues. In order to produce seedlings of good quality, taking into account the composition and availability of substrates in the region, the present study aimed to evaluate the influence of the application of different doses of biochar produced from poultry litter as a substrate conditioner for the production of fruit trees.