Animals are very important part of our biodiversity. They are the living part of our colorful nature and have always fascinated human imaginations and figure abundantly in literature and art and craft. Pakistan has 188 species of mammals, 670 species of birds, 185 species of reptiles, and 22 species of amphibians. In this study, biology and behavior of Nilgai, Chinkara, Hog Deer, Blackbuck and Chital was studied at the Safari Park and Karachi Zoo. It was observed that captive animals develop different kind of behaviour depending upon the environment provided. Safari Parks and Zoos have a capability to become centers for animal behavior. The research techniques and information are helpful for graduate and research students of wildlife, National Parks managers, wildlife biologists, wildlife conservation organizations etc.