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In this work, we have set as an objective the improvement of the conservation of the sperm of some species, namely: Bovine, avian and rabbit, this through an intermediate objective; the improvement of the solubility of vitamin E and cholesterol in aqueous medium. For this purpose we prepared several complexes that we characterized by IR and DRX, and that we applied to a cellular model (spermatozoon). The results obtained show that we were able to improve the solubility of vitamin E and cholesterol, using cyclodextrins and PEG 6000 on the one hand, and to discover a synergistic effect between vitamin E and cholesterol in their complexed and dispersed forms on sperm conservation on the other hand.