Subcellular Fractionation: A Laboratory Manual
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The successful isolation of specific cellular structures permits in-depth studies of their function and composition. This laboratory manual provides step-by-step protocols for the extraction of subcellular components from animal tissues, yeasts, plants, and cultured cells. Each chapter describes methods for isolating a particular organelle, vesicle, membrane, or macromolecular complex. Strategies for breaking cells without damaging the component of interest, enriching for that component based on its physical and biochemical characteristics, and monitoring and ensuring the success of the purification procedure are provided.…mehr

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Produktbeschreibung
The successful isolation of specific cellular structures permits in-depth studies of their function and composition. This laboratory manual provides step-by-step protocols for the extraction of subcellular components from animal tissues, yeasts, plants, and cultured cells. Each chapter describes methods for isolating a particular organelle, vesicle, membrane, or macromolecular complex. Strategies for breaking cells without damaging the component of interest, enriching for that component based on its physical and biochemical characteristics, and monitoring and ensuring the success of the purification procedure are provided.
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Autorenporträt
Paul Pryor is a newly appointed lecturer in the Centre for Immunology and Infection (CII) and HYMS. Paul obtained his BSc in Biochemistry from the University of York and then went on to do a PhD in Cell Biology in the laboratory of Professor Geoff Holman, University of Bath. Paul then undertook post-doctoral research in the laboratory of Professor J. Paul Luzio, University of Cambridge, researching the molecular mechanisms in delivery of material to lysosomes. In 2005 Paul was awarded a Wellcome Trust Career Development Fellowship and moved to the Department of Biology (York) in 2007, investigating phagolysosome biogenesis.