This volume summarizes the new developments that made subcellular proteomics a rapidly expanding area. It examines the different levels of subcellular organization and their specific methodologies. In addition, the book includes coverage of systems biology that deals with the integration of the data derived from these different levels to produce a synthetic description of the cell as a system.
This volume summarizes the new developments that made subcellular proteomics a rapidly expanding area. It examines the different levels of subcellular organization and their specific methodologies. In addition, the book includes coverage of systems biology that deals with the integration of the data derived from these different levels to produce a synthetic description of the cell as a system.
Membrane Proteomics.- Keynotes on Membrane Proteomics.- Two-Dimensional BAC/SDS-PAGE for Membrane Proteomics.- Microparticles: A New Tool for Plasma Membrane Sub-cellular Proteomic.- Lipid Raft Proteomics: More than Just Detergent-Resistant Membranes.- Organelle Subproteomes.- Organelle Proteome Variation Among Different Cell Types: Lessons from Nuclear Membrane Proteins.- Synaptosome Proteomics.- Proteomic Analysis of Secreted Exosomes.- Characterization of Supramolecular Protein Complexes.- From Protein-Protein Complexes to Interactomics.- Supramolecular Signalling Complexes in the Nervous System.- Protein Networks and Complexes in Photoreceptor Cilia.- Subcellular Systems Biology.- Systems Biology and the Reconstruction of the Cell: From Molecular Components to Integral Function.- Automated, Systematic Determination of Protein Subcellular Location using Fluorescence Microscopy.- Systems Biology of the Endoplasmic Reticulum Stress Response.- Emerging Technologies in Proteomics.- Systems Nanobiology: From Quantitative Single Molecule Biophysics to Microfluidic-Based Single Cell Analysis.- Biophotonics Applied to Proteomics.- Differential Epitope Identification of Antibodies Against Intracellular Domains of Alzheimer's Amyloid Precursor Protein Using High Resolution Affinity-mass Spectrometry.- LC-MALDI MS and MS/MS - An Efficient Tool in Proteome Analysis.
Membrane Proteomics.- Keynotes on Membrane Proteomics.- Two-Dimensional BAC/SDS-PAGE for Membrane Proteomics.- Microparticles: A New Tool for Plasma Membrane Sub-cellular Proteomic.- Lipid Raft Proteomics: More than Just Detergent-Resistant Membranes.- Organelle Subproteomes.- Organelle Proteome Variation Among Different Cell Types: Lessons from Nuclear Membrane Proteins.- Synaptosome Proteomics.- Proteomic Analysis of Secreted Exosomes.- Characterization of Supramolecular Protein Complexes.- From Protein-Protein Complexes to Interactomics.- Supramolecular Signalling Complexes in the Nervous System.- Protein Networks and Complexes in Photoreceptor Cilia.- Subcellular Systems Biology.- Systems Biology and the Reconstruction of the Cell: From Molecular Components to Integral Function.- Automated, Systematic Determination of Protein Subcellular Location using Fluorescence Microscopy.- Systems Biology of the Endoplasmic Reticulum Stress Response.- Emerging Technologies in Proteomics.- Systems Nanobiology: From Quantitative Single Molecule Biophysics to Microfluidic-Based Single Cell Analysis.- Biophotonics Applied to Proteomics.- Differential Epitope Identification of Antibodies Against Intracellular Domains of Alzheimer's Amyloid Precursor Protein Using High Resolution Affinity-mass Spectrometry.- LC-MALDI MS and MS/MS - An Efficient Tool in Proteome Analysis.
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