Super-Resolution Imaging in Biomedicine

Herausgeber: Diaspro, Alberto; Zandvoort, Marc A. M. J. van

• Gebundenes Buch

Produktbeschreibung

This book encompasses the full breadth of the super-resolution imaging field, representing modern techniques that exceed the traditional diffraction limit, thereby opening up new applications in biomedicine. It shows readers how to use the new tools to increase resolution in sub-nanometer-scale images of living cells and tissue, which leads to new information about molecules, pathways and dynamics. The book highlights the advantages and disadvantages of the techniques, and gives state-of-the-art examples of applications using microscopes currently available on the market.

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Produktdetails

• Verlag: CRC Press
• Seitenzahl: 448
• Erscheinungstermin: 14. Oktober 2016
• Englisch
• Abmessung: 260mm x 183mm x 29mm
• Gewicht: 1034g
• ISBN-13: 9781482244342
• ISBN-10: 1482244349
• Artikelnr.: 47367687

Autorenporträt

Alberto Diaspro, Ph.D, is a professor of applied physics and head of the Nanophysics Unit of the Italian Institute of Technology. He also founded the Laboratory for Advanced Microscopy, Bioimaging, and Spectroscopy (LAMBS). Marc A.M.J. van Zandvoort, Ph.D, is the leader of the Two-Photon Core Facility in Aachen and head of the Advanced Microscopy Unit in the Department of Genetics and Cell Biology at Maastricht University, Netherlands.

Inhaltsangabe

Overview of Super-Resolution Technologies. Introduction to the Main
Techniques - (F)PALM/(d)STORM, STED, SIM. Super-Resolution Imaging of
Single Molecules. Image Processing for Super-Resolution. Super-Resolution
Fluorescent Probes. The Methods. Principles and Techniques for STED;
SIM/SSIM; SOFI. Single-Molecule Localization Microscopy - PALM, (F)PALM,
(d)STORM): Principles, Probes, and Algorithms. Principles and Techniques
for SPDM; GSDIM. Applications in Biology and Medicine. Synaptic Protein
Distributions Studied with (d)STORM/(F)PALM. Studies of Chromosomal
Organization at Super Resolution. Nanoscale Organization of RNA Polymerase
Using PALM. Super-Resolution Microscopy of Protein Machines in Bacteria.
Super-Resolution Microscopy of Living Biological Nanostructures. SIM for
the Study of Mitochondrial Dynamics in 3D. Neurobiological Applications
Using STED. STED in HIV Research. Super-Resolution Imaging in
Cardiovascular Research. Quantitative Super-Resolution Imaging of Proteins.