This book introduces the fundamentals, instruments, methodology, and applications of surface plasmon resonance imaging (SPRi) and related techniques. It provides an overview of SPRi development and an easy-to-understand interpretation of theory and operation principles. Some unique ideas proposed by the authors to design and set up SPRi devices and methods are disclosed for the first time. Crucial manipulation experiences are also summarized here, including chip surface functionalization, sensitivity enhancement and coupling of SPRi with other analytical techniques. The application of SPRi for…mehr
This book introduces the fundamentals, instruments, methodology, and applications of surface plasmon resonance imaging (SPRi) and related techniques. It provides an overview of SPRi development and an easy-to-understand interpretation of theory and operation principles. Some unique ideas proposed by the authors to design and set up SPRi devices and methods are disclosed for the first time. Crucial manipulation experiences are also summarized here, including chip surface functionalization, sensitivity enhancement and coupling of SPRi with other analytical techniques. The application of SPRi for molecular interaction study, featuring high throughput, label-freeness, and physiologically compatible analysis, is discussed in detail. This book is of interest and useful to a wide readership in bioanalytical chemistry, molecular biology, and many related interdisciplinary fields.
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Autorenporträt
Yi Chen obtained his MS and Ph.D. from Institute of Chemistry, Chinese Academy of Sciences, in 1987 and 1990, respectively, and then worked in the same institute as Research Assistant and was promoted to Associate Professor in 1991 and Full Professor in 1995. At present, he is also a Professor at University of Chinese Academy of Science, and at Huiyin Institute of Technology; Associate/Deputy Editor of Chinese Journal of Analytical Chemistry, Chinese Journal of Chromatography, and Electrophoresis; and Vice Chairman of Science Popularization Work Committee, China Instrument and Control Society. Dr. Chen started to work on capillary electrophoresis in 1984, surface plasmon resonance and imaging in 1997, and mass spectrometry in 2004. He is author and co-author of 332 journal articles and 3 books, and holds more than 45 patents.
Inhaltsangabe
Introduction.- Basic Theory.- Instrumentation.- Methodology.- Interaction and Reaction.- Analysis of Molecules and Biomolecules.
