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There is a lack of knowledge of the presence of the state of stress during an educational assessment of skills and knowledge in the field of surgical instrumentation and nursing. By conducting a prospective, observational research with qualitative and quantitative analysis, it was indirectly demonstrated the presence of the state of stress during an evaluation of knowledge and skills of the actual clinical practice, without prior analysis of the psychological traits of the students involved. It is a preliminary study that, nevertheless, puts into discussion the real interference of the stress state during a practical evaluation of competences.