This book provides a convenient and simple method for the synthesis of novel pyrazole substituted 9-anilinoacridine derivatives. The synthesized compounds were analyzed and confirmed by physical and analytical data. The synthesized compounds were evaluated for their antimicrobial activity, invitro antioxidant activity and anticancer activity against HEp-2 cell line. The users of this book will be provided with the synthetic routes of pyrazole substituted 9-anilinoacridine derivatives and their biological screening methods for antimicrobial activity, invitro antioxidant activity and anticancer activity.