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Series of substituted Synthesis of sub -N-sub-1,3,4-thiadiazol-2-amine derivatives (3a-t) containing 1,3,4 - thiadiazol moiety were designed and synthesized by conventional and microwave as a green chemistry method. Compound 3b showed potent activity against potent inhibitors of NUDTs silence hormone signaling in breast cancer (PDB Code-5NQR) for anti-cancer activity and 3b showed potent activity against receptor Crystal structure of Rhodostomin ARLDDL mutant (PDB Code-3UCI) for antibacterial activity, Compound 3f showed potent activity against Snapshots of the RNA processing factor SCAF8 bound to different phosphorylated forms of the Carboxy-Terminal Domain of RNA-Polymerase II (PDB Code-3D9K) for anti-inflammatory activity. Activity of these synthesized 3a t compounds has been verified by IR spectral characterization and IR, NMR and mass of compound 3b, 3f and 3r has been established. Result indicated that these compounds showed promising anticancer activity, antibacterial activity and anti-inflammatory activity in comparison to standard drugs was used.