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This study deals with the synthesis of SeNPs using EWL and ascorbic acid for the generation of metal nanoparticles. The solvents and reducing agents determine the physical and morphological characteristics of SeNPs. The size, con-formation and morphological characteristics of the newly synthesized nanoparticles were studied by UV-Vis, FT-IR, XRD and TEM analyses. The EWL stability dispersions of SeNPs were observed to exhibit prolonged stability mini-mum 12 months, which was confirmed by the TEM image. The antibacterial efficacies of the reported SeNPs stabilized with EWL have been proved to be promising against different pathogenic bacteria. Hence, further studies are to be conducted in this research area to be employed in the pharmaceutical industry, and this would be a cost-effective alternative to other approaches.