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In this book we have established a novel series of 2-(2-chlorophenyl)quinoline-4-carboxylic acid based thiosemicarbazide derivatives all compounds are conforming by their physical data (melting point) and spectral data (Mass analysis , 1H NMR, 13C NMR). All compounds are analyzing in antibacterial and antifungal Biological activity. In biological analysis we have found that many compounds have potent activity against different strains.