Synthesis of tyrosinase by streptomyces SP. - H, Raghavendra
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- In all 15 isolates of Streptomyces were screened for the synthesis of tyrosinase by both qualitative and quantitative methods in synthetic medium. - Activity of tyrosinase, specific activity of tyrosinase, formation of melanin and protein content of tyrosinase were assessed to determine the catalytic power of tyrosinase. - In a batch wise bioprocess, an isolate of Streptomyces DLS-8 has been found to be potential for the production of tyrosinase. - Effect of pH and temperature on the production of tyrosinase were also examined. 300C temperature and pH 8.5 were proved to be optimum for the…mehr

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Produktbeschreibung
- In all 15 isolates of Streptomyces were screened for the synthesis of tyrosinase by both qualitative and quantitative methods in synthetic medium. - Activity of tyrosinase, specific activity of tyrosinase, formation of melanin and protein content of tyrosinase were assessed to determine the catalytic power of tyrosinase. - In a batch wise bioprocess, an isolate of Streptomyces DLS-8 has been found to be potential for the production of tyrosinase. - Effect of pH and temperature on the production of tyrosinase were also examined. 300C temperature and pH 8.5 were proved to be optimum for the maximum production of tyrosinase. - Results of the present study revealed that starch and citrulline were the important carbon and nitrogen sources respectively for the maximum production of tyrosinase. - The partial isolation and purification of the tyrosinase was under taken by SDS-PAGE. The tyrosinase was determined to be about 45 KDa, comparing to BSA as standard marker.- Liquid chromatogram mass spectrum reveals the presence of tyrosine, L-Dopa and melanin based on their base peaks for their respective molecular weights indicating significantly the catalytic power of tyrosinase.
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Autorenporträt
Dr Raghavendra H, is completed his M.Sc., M.Phil., Ph.D., in Microbiology, He did Post-Doctoral Research Under the supervision of Prof. Dayanad Agasar, Vice chancellor of Gulbarga University. He is expertise in the synthesis of tyrosinase using streptomyces sp. Currently working as faculty of Microbiology at Raichur University, Raichur.