Silvia Pimentel, Rui Gomes, Laurent Fasano

Teashirt in cell proliferation

Analysis of tsh mutants in Drosophila melanogaster

• Broschiertes Buch

Produktbeschreibung

Here we present evidence that the transcription factor Teashirt (Tsh) has a role in cell proliferation in Drosophila follicle stem cells (FSC), neuroblasts and S2 cells. In ovaries, Tsh and Hedgehog (Hh) are co-expressed in the terminal filament and cap cells. The characterisation of a new tsh mutation causing female sterility allowed us to show that Tsh acts via Hh-mediated signalling pathway to control FSC proliferation. In this tsh background, the hsGal4 driven expression of tsh or hh partially rescues the abnormal phenotype allowing oogenesis progression. Furthermore, tsh overexpression mimics the hh overexpression associated phenotype. In neuroblasts, mutant tsh alleles are unable to exit mitosis. The phenotypes of double mutants with rod or bub1 resemble the tsh single mutant phenotype, suggesting failure to escape mitosis. Finally, tsh depletion in S2 cells mimics the tsh mutant phenotype in neuroblasts. We propose that during oogenesis, tsh affects FSC proliferation mediating Hh expression. In neuroblasts, tsh may also acts through Hh signalling, since expression of interference hedgehog, was severely downregulated in our DNA microarray analysis.

Produktdetails

• Verlag: LAP Lambert Academic Publishing
• Aufl.
• Seitenzahl: 200
• Erscheinungstermin: 21. März 2012
• Englisch
• Abmessung: 220mm x 150mm x 12mm
• Gewicht: 316g
• ISBN-13: 9783848428502
• ISBN-10: 3848428504
• Artikelnr.: 35347525

Autorenporträt

Dr. Silvia Pimentel has obtained her PhD degree in 2011, here is shown her PhD results. Dr. Rui Gomes (PhD degree in 1994) Professor at Lisbon University (FCUL) works in the fields of Cell Cycle Regulation and Neurosciences. Dr. Laurent Fasano (PhD degree in 1990) CNRS Researcher at the IBDML works in the field of Developmental Biology.