Successful root canal treatment depends upon many factors, one of the most important factors is removal of micro-organisms through bio-mechanical instrumentation of the root canal system which includes removal of infected dentin and organic tissue by cleaning and shaping. This is done by enlarging and shaping the canal to allow for adequate chemical debridement, while preserving the radicular anatomy. A lot of techniques have been developed over the past few decades. This book tries to provide comprehensive knowledge regarding various techniques of bio-mechanical preparation. It focuses on history, all the instrumentation techniques in detail with diagrams, controversies regarding instrumentation and its recent advances. The book has easy to read student friendly chapters for their better understanding. A good understanding and abiding love for the subject has been given into the making of this book.