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Over the past 30 years, the scientific world has witnessed a dramaticinterest in phytochemistry with research contribution from biologist,chemist, biochemist and pharmacist as well as scientist to discover anewly drugs through ethno-botanical route. Students and researchers'need manual book contain all qualitative and quantitative tests to detectprimary and secondary metabolites from medicinal plants which havedifferent pharmacological and biological effects against differentaliments.