This book will be helpful to UG & PG students of Pharmacy as well as Analytical Chemistry students.This text book deals with the basic study of the important topic of Pharmaceutical Analysis subject consist of the detailed Chromatography, it's theory, types of column chromatography and Advanced column chromatography Instrumentation, Applications of HPLC,and UPLC. The process of Calibration & Validation of Instrument HPLC. The procedure carried out in New method development and validation by HPLC. I hope this book will useful for the study of students as well as researchers.