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The gradual depletion of the petroleum reserves and the increasing environmental concerns has created a great demand for alternative source of petroleum based fuel. Energy consumption, economic growth, population growth and industrialization have led to higher energy demand thus, leading to find out the best candidate for a fossil fuel substitution, which should provide advancement and benefits economic and environmental demands. Considering the need of alternative fuel, environmental concerns and current trend of research activities the target-oriented research and development activities on biodiesel will be the trust area to continue. With reference to the search for potent non-edible feedstock this book is focused on technical and economic feasibility of tree borne oil seeds with respect to its bioproductivity, quality of biodiesel and molecular characterisation of gender specific gene by utilising molecular techniques.