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The scope of this Special Issue is to collect some of the contributions to the 2nd International Electronic Conference on Biosensors, which was held to gather well-known experts worldwide who are currently working in the field of biosensors and provide an online forum for sharing and exchanging knowledge on this subject. Biosensors have a very wide range of applications at present, due to their simplicity, low cost and precision, which aim to improve the quality of life. This range covers their use for environmental monitoring, disease detection, food safety, defense, drug discovery, etc. Particular attention was oaid to these different topics, including implantable and wearable biosensors, the role of biological and chemical recognition elements, microfluidics and point-of-care testing.