Fakhri Mesri & Fereshteh Deyhim, Department of Humanities, Payam-e-Noor University, Tehran, I.R of IRAN. The purpose of this present study aims to investigate the relationship between gender, proficiency level and Iranian EFL learners' performance on pre-listening activities. 90 Iranian EFL learners' participated in the present study. They received three different types of listening support which were (question preview, repeated listening, and pre-teaching vocabulary). Language proficiency test was administrated to divide them in to high and low level groups. Also there were three experimental groups. The first experimental group received pre-question activity, the second experimental group received repeated listening and the third experimental group received pre-teaching vocabulary. Data analysis has been done with Statistical Package for Social Sciences (SPSS) software. T test, One-Way ANOVA and Post Hoc were used to analyze the collected data.The results showed that Question preview was the most helpful support to different gender and proficiency levels.