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Present study is designed to record the effects of FSH present in the extract obtained from culture of buffalo adenohypophysis on the serum hormonal profile and ovarian picture in Nili-Ravi buffaloes. For this purpose, nine normal health and cyclic buffaloes of Nili-Ravi breed with normal reproductive tract was kept at livestock farm, University of Agriculture Faisalabad. They were divided in three groups. The results of the analysis are shown further in the book.