A Students who has just entered the portals of colleges finds it difficult to understand the research taught to him/her this difficulty is mainly due to understand standard references. While preparing this book "PHYSIO-CHEMICAL ANALYSIS IN SELECTED SITES AND MOLECULAR STUDIES WITH CONSERVATION STATUS OF FRESH WATER STREAM FISHES IN PALANI HILLS OF SOUTHERN INDIA "has been prepared to serve the special purpose of research students. This book explains the basic research methodology and Techniques in a simplified manner. This book can be used for PG and research scholar of Zoology students, who are looking for a quick revision before their research.