The Nuclear Receptor Superfamily

Methods and Protocols
Herausgegeben:McEwan, PhD, Iain J.

• Broschiertes Buch

Produktbeschreibung

Thisvolume aims to describe acomplementary range of molecular, cell biological, and in vivo protocols used to investigatethe structure-function of nuclear receptors, together with experimentalapproaches that may lead to new drugs to selectively target nuclearreceptor-associated diseases. The NuclearReceptor Superfamily, Second Edition will benefit those starting out in thenuclear receptor research field as well as to more established researchers whowish to apply different methods to a particular receptor or research problem.Written in the highly successful Methods in Molecular Biology seriesformat, chapters include introductions to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritativeand cutting-edge, The Nuclear ReceptorSuperfamily, Second Edition aims to ensure successful results in thefurther study of this vital field.

Produktdetails

• Methods in Molecular Biology 1443
• Verlag: Humana / Springer New York / Springer, Berlin
• Artikelnr. des Verlages: 978-1-4939-8120-5
• 2. Aufl.
• Seitenzahl: 260
• Erscheinungstermin: 26. Mai 2018
• Englisch
• Abmessung: 254mm x 178mm x 15mm
• Gewicht: 512g
• ISBN-13: 9781493981205
• ISBN-10: 149398120X
• Artikelnr.: 52906911

Inhaltsangabe

The Nuclear Receptor Superfamily- AtThirty.- Lipid Homeostasis and Ligands forLiver X Receptors: Identification and Characterization.- Useof Differential Scanning Fluorimetry to Identify Nuclear Receptor Ligands.- Drug-Discovery Pipeline for NovelInhibitors of the Androgen Receptor.- Useof BRET to Study Protein-Protein Interactions in vitro and in vivo.- StudyingSteroid Receptor Complexes in the Cellular Environment.- Post-translationalModifications of Steroid Receptors: Phosphorylation.- Mapping protein-DNA Interactions using ChIP-exo and Illumina-based Sequencing.- Methods to IdentifyChromatin Bound Protein Complexes: From Genome-Wide to Locus Specific Approaches.- Measuring the Expression of microRNAs Regulated byAndrogens.- Methods for Identifying andQuantifying Mrna Expression of Androgen Receptor Splicing Variants in ProstateCancer.- HarvestingHuman Prostate Tissue Material and Culturing Primary Prostate Epithelial Cells.- Invivo Imaging of Nuclear Receptor Transcriptional Activity.- Developmentand Characterisation of Cell-Specific Androgen Receptor Knockout Mice.