This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers recent research and methods development for changing the DNA sequence within the genomes of cells and organisms. Focusing on enzymes that generate double-strand breaks in DNA, the chapters describe use of molecular tools to introduce or delete genetic information at specific sites in the genomes of animal, plant and bacterial cells.
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers recent research and methods development for changing the DNA sequence within the genomes of cells and organisms. Focusing on enzymes that generate double-strand breaks in DNA, the chapters describe use of molecular tools to introduce or delete genetic information at specific sites in the genomes of animal, plant and bacterial cells.
1. In Vitro Enzymology of Cas9 Carolin Anders and Martin Jinek 2. Targeted Genome Editing in Human Cells Using Crispr-Cas Nucleases and Truncated Guide Rnas Yanfang Fu, Deepak Reyon and J. Keith Joung 3. Determining the Specificities of Talens, Cas9, and Other Genome Editing Enzymes Vikram Pattanayak , John P. Guilinger,and David R. Liu 4. Genome Engineering with Custom Recombinases Thomas Gaj, and Carlos F. Barbas 5. Genome Engineering in Human Cells Minjung Song, Young-Hoon Kim, Hyongbum Kim1 and Jin-Soo Kim 6. Genome Editing in Human Stem Cells Susan M. Byrne, Prashant Mali, and George M. Church 7. Tagging Endogenous Loci for Live-Cell Fluorescence Imaging and Molecule Counting Using ZFNs, Talens and Cas9 Dambournet D., Hong SH. , Grassart A and Drubin DG 8. Genome Editing Using Cas9 Nickases Alexandro E. Trevino and Feng Zhang 9. Assaying Break and Nick-Induced Homologous Recombination in Mammalian Cells Using the DR-GFP Reporter and Cas9 Nucleases Lianne E.M. Vriend , Maria Jasin and Przemek M. Krawczyk 10. Adapting CRISPR/Cas9 for Functional Genomics Screens Abba Malina , Alexandra Katigbak, Regina Cencic, Rayelle Itoua Maïga, Francis Robert, Hisashi Miura and Jerry Pelletier 11. The iCRISPR Platform for Rapid Genome Editing in Human Pluripotent Stem Cells Zengrong Zhu , Federico González and Danwei Huangfu 12. Creating Cancer Translocations in Human Cells Using Cas9 DSBs and nCas9 Paired Nicks Benjamin Renouf, Marion Piganeau, Hind Ghezraoui, Maria Jasin and Erika Brunet 13. Genome Editing for Human Gene Therapy Torsten B. Meissner, Pankaj K. Mandal, Leonardo M. R. Ferreira, Derrick J. Rossi and Chad A. Cowa 14. Generation of Site-Specific Mutations in the Rat Genome Via CRISPR-Cas9 Yuting Guan, Yanjiao Shao, Dali Li and Mingyao Liu 15. CRISPR-Cas9-Based Genome Editing in Mice by Single Plasmid Injection Yoshitaka Fujihara and Masahito Ikawa 16. Imaging Genomic Elements in Living Cells using CRISPR/Cas9 Baohui Chen and Bo Huang 17. Cas9-Based Genome Editing in Xenopus Tropicalis Takuya Nakayama, Ira L. Blitz, Margaret B. Fish, Akinleye O. Odeleye, Sumanth Manohar, Ken W.Y. Cho and Robert M. Grainger 18. Cas9-Based Genome Editing in Zebrafish Andrew P.W. Gonzales, and Jing-Ruey Joanna Yeh 19. Cas9-Based Genome Editing in Drosophila Benjamin E. Housden , Shuailiang Lin and Norbert Perrimon 20. Transgene Free Genome Editing by Germline Injection of CRISPR Cas RNA Hillel T Schwartz and Paul W Sternberg 21. Cas9-Based Genome Editing in Arabidopsis and Tobacco Jian-Feng Li, Dandan Zhang, and Jen Sheen 22. Multiplex Engineering of Industrial Yeast Genomes Using CRISPRm Owen W. Ryan1 and Jamie H. D. Cate 23. Protein Engineering of Cas9 for Enhanced Function Benjamin L. Oakes, Dana C. Nadler, and David F. Savage
1. In Vitro Enzymology of Cas9 Carolin Anders and Martin Jinek 2. Targeted Genome Editing in Human Cells Using Crispr-Cas Nucleases and Truncated Guide Rnas Yanfang Fu, Deepak Reyon and J. Keith Joung 3. Determining the Specificities of Talens, Cas9, and Other Genome Editing Enzymes Vikram Pattanayak , John P. Guilinger,and David R. Liu 4. Genome Engineering with Custom Recombinases Thomas Gaj, and Carlos F. Barbas 5. Genome Engineering in Human Cells Minjung Song, Young-Hoon Kim, Hyongbum Kim1 and Jin-Soo Kim 6. Genome Editing in Human Stem Cells Susan M. Byrne, Prashant Mali, and George M. Church 7. Tagging Endogenous Loci for Live-Cell Fluorescence Imaging and Molecule Counting Using ZFNs, Talens and Cas9 Dambournet D., Hong SH. , Grassart A and Drubin DG 8. Genome Editing Using Cas9 Nickases Alexandro E. Trevino and Feng Zhang 9. Assaying Break and Nick-Induced Homologous Recombination in Mammalian Cells Using the DR-GFP Reporter and Cas9 Nucleases Lianne E.M. Vriend , Maria Jasin and Przemek M. Krawczyk 10. Adapting CRISPR/Cas9 for Functional Genomics Screens Abba Malina , Alexandra Katigbak, Regina Cencic, Rayelle Itoua Maïga, Francis Robert, Hisashi Miura and Jerry Pelletier 11. The iCRISPR Platform for Rapid Genome Editing in Human Pluripotent Stem Cells Zengrong Zhu , Federico González and Danwei Huangfu 12. Creating Cancer Translocations in Human Cells Using Cas9 DSBs and nCas9 Paired Nicks Benjamin Renouf, Marion Piganeau, Hind Ghezraoui, Maria Jasin and Erika Brunet 13. Genome Editing for Human Gene Therapy Torsten B. Meissner, Pankaj K. Mandal, Leonardo M. R. Ferreira, Derrick J. Rossi and Chad A. Cowa 14. Generation of Site-Specific Mutations in the Rat Genome Via CRISPR-Cas9 Yuting Guan, Yanjiao Shao, Dali Li and Mingyao Liu 15. CRISPR-Cas9-Based Genome Editing in Mice by Single Plasmid Injection Yoshitaka Fujihara and Masahito Ikawa 16. Imaging Genomic Elements in Living Cells using CRISPR/Cas9 Baohui Chen and Bo Huang 17. Cas9-Based Genome Editing in Xenopus Tropicalis Takuya Nakayama, Ira L. Blitz, Margaret B. Fish, Akinleye O. Odeleye, Sumanth Manohar, Ken W.Y. Cho and Robert M. Grainger 18. Cas9-Based Genome Editing in Zebrafish Andrew P.W. Gonzales, and Jing-Ruey Joanna Yeh 19. Cas9-Based Genome Editing in Drosophila Benjamin E. Housden , Shuailiang Lin and Norbert Perrimon 20. Transgene Free Genome Editing by Germline Injection of CRISPR Cas RNA Hillel T Schwartz and Paul W Sternberg 21. Cas9-Based Genome Editing in Arabidopsis and Tobacco Jian-Feng Li, Dandan Zhang, and Jen Sheen 22. Multiplex Engineering of Industrial Yeast Genomes Using CRISPRm Owen W. Ryan1 and Jamie H. D. Cate 23. Protein Engineering of Cas9 for Enhanced Function Benjamin L. Oakes, Dana C. Nadler, and David F. Savage
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