In Basrah ,people suffer from Leishmaniasis caused by Leishmania donovani and Leishmania tropica. It was noted in the recent increase in the incidence of the disease in many parts of the world including Iraq due to the emergence of new strains of chemical drug-resistant parasite or that chemicals drugs do not work immediately on parasite. So use of alternatives therapeutic for the treatment of leishmaniasis including medicinal plants in Basrah Hibiscus sabdariffa flowers and Helianthus annus which extracted from it glycosides called vicine beside of test effects therapitic of cisplatine against Leishmania donovani also Studying effecte of using culture media and vitamins as stimulater growth for Leishmania donovani. The fructose was more activity in increase number of promastigote in vitro