Tatyana Svinkina

TOR and GSK3 in the ciliate Paramecium tetraurelia

Target of Rapamycin and Glycogen Synthase Kinase 3 pathway orthologs and their regulation of ciliary length/assembly

• Broschiertes Buch

Produktbeschreibung

Target of rapamycin (TOR) is a serine threonine kinase that regulates cellular processes in response to the stresses and nutrients in the environment. We investigated TOR signaling pathway in the ciliate Paramecium tetraurelia (P. tetraurelia). Using BLAST (Basic Local Alignment Search Tool) we identified orthologs of the mammalian TOR, LST8, Akt, Rheb, Rag A/B, Rag C/D, SNAT2, Tap42, S6K, PKA and GSK3 in the P. tetraurelia. With RNA interference technique we established that depletion of these orthologs reduced cellular proliferation and arrested cells between G1 and S stages of the cell cycle. Furthermore, GSK3 depletion produced round looking cells with short, sparse cilia, and GSK3 was localized to the pellicle and cilia of P. tetraurelia. In addition to these investigations, we determined that inhibition of PKA with H89 prevented re-growth of cilia in de-ciliated cells. Our results possibly suggest that GSK3 and PKA work together in the regulation of ciliary length and /or assembly in P. tetraurelia. We also hypothesize that these regulations are TOR dependent.

Produktdetails

• Verlag: LAP Lambert Academic Publishing
• Aufl.
• Seitenzahl: 340
• Erscheinungstermin: 30. Mai 2012
• Englisch
• Abmessung: 220mm x 150mm x 21mm
• Gewicht: 524g
• ISBN-13: 9783659128356
• ISBN-10: 365912835X
• Artikelnr.: 36000538

Autorenporträt

I have received my graduate degree in cell and molecular biology at the University of Vermont, in the laboratory of Dr. Judith Van Houten, where I investigated Mammalian Target of Rapamycin signaling pathways. Presently, I work as a researcher for the proteomics group at the Broad Institute of MIT and Harvard.