Towards Clearer Imaging of Luminescent and Bioluminescent Samples - Mader, Kevin
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Bioluminescence has become an excellent method for tagging and easily identifying biochemical processes. The process produces light from a set of biochemical reactions such that a light source is not needed and imaging can be done in regions a light source could not easily be placed. Also larger samples do not require any scanning, as other techniques like both confocal and two-photon. The major issue with the technique is that because it does not require illumination, the resulting images are usually blurry and poorly sectioned. In this thesis a series of optical and computational techniques…mehr

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Produktbeschreibung
Bioluminescence has become an excellent method for tagging and easily identifying biochemical processes. The process produces light from a set of biochemical reactions such that a light source is not needed and imaging can be done in regions a light source could not easily be placed. Also larger samples do not require any scanning, as other techniques like both confocal and two-photon. The major issue with the technique is that because it does not require illumination, the resulting images are usually blurry and poorly sectioned. In this thesis a series of optical and computational techniques are used to take advantage of the propagation of phase rather than intensity to reject significantly more light from out-side the focal region.
Autorenporträt
Kevin Mader studied Electrical Engineering and Photonics at Boston University specializing in biomedical applications. During his studies, he did a summer fellowship at the Center for Biophotonics at the University of California Davis, and an international research exchange at the Department of Physics at Universität Bielefeld in Germany.