Alan R. Clarke (ed.)

Transgenesis Techniques

Principles and Protocols
Herausgeber: Clarke, Alan R.

• Gebundenes Buch

Produktbeschreibung

This revised and updated edition of a much-acclaimed classic presents a collection of readily reproducible techniques for the generation and analysis of transgenic animals, with emphasis on the manipulation of the mammalian genome. It concentrates on the creation and maintenance of genetically modified murine strains, providing access to the germline by both conventional pronuclear injection, and by retroviral and adenoviral infection. Extensive coverage is also given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for both constitutive and conditional (Cre-lox or the Flp-frt systems) gene targeting. Additional chapters provide cutting-edge techniques for the cryopreservation of both male and female germlines, for the generation of transgenic sheep by nuclear transfer, and for the basic analysis of transgenic organisms.

Produktdetails

• Methods in Molecular Biology 180
• Verlag: Springer, Berlin
• 2nd ed.
• Seitenzahl: 368
• Englisch
• Abmessung: 229mm x 152mm
• Gewicht: 1g
• ISBN-13: 9780896036963
• ISBN-10: 0896036960
• Artikelnr.: 21415931

Inhaltsangabe

"Part I. Topical Reviews in Transgenesis

Biomedical and Agricultural Applications of Animal Transgenesis
Jim McWhir

Part II. Transgenesis in Invertebrate and Lower Vertebrate Species

Gene Transfer in Drosophila
Mark J. O'Connor and William Chia

Part III. Transgenesis in the Mouse: Oocyte Injection

Oocyte Injection in the Mouse
Gary A. J. Brown and Timothy J. Corbin

Part IV. Alternative Routes to the Germline

Adenoviral Infection
Tohru Tsukui and Yutaka Toyoda

Retroviral Infection
Richard A. Bowen

Part V. Transgenesis in the Mouse: The ES Cell Route

In Vitro Isolation of Murine Embryonic Stem Cells
David Wells

Production of Chimeras Derived from Murine Embryonic Stem Cells
David Wells

Gene Targeting Strategies
David W. Melton

Cre/loxP Recombination System and Gene Targeting
Ralf Kühn and Raul M. Torres

Part VI. Cryopreservation of Mouse Lines

Cryopreservation of Transgenic Mouse Lines
Jillian M. Shaw and Naomi Nakagata

Ovarian Tissue Transplantation and Cryopreservation: Application to Maintenance and Recovery of Transgenic and Inbred Mouse Lines
Jillian M. Shaw and A. O. Trounson

Part VII. Transgenesis in the Rat

Transgenesis in the Rat
Linda J. Mullins, Gillian Brooker, and John J. Mullins

Part VIII. Transgenesis in Domestic Species

Generation of Transgenic Livestock by Pronuclear Injection
A. John Clark

Transgenic Sheep from Cultured Cells
Keith H. S. Campbell

Part IX. Characterization and Analysis of Transgenic Strains

Analysis of Transgenic Mice
Stefan Selbert and Dominic Rannie

Index."