This revised and updated edition of a much-acclaimed classic presents a collection of readily reproducible techniques for the generation and analysis of transgenic animals, with emphasis on the manipulation of the mammalian genome. It concentrates on the creation and maintenance of genetically modified murine strains, providing access to the germline by both conventional pronuclear injection, and by retroviral and adenoviral infection. Extensive coverage is also given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for both constitutive and…mehr
This revised and updated edition of a much-acclaimed classic presents a collection of readily reproducible techniques for the generation and analysis of transgenic animals, with emphasis on the manipulation of the mammalian genome. It concentrates on the creation and maintenance of genetically modified murine strains, providing access to the germline by both conventional pronuclear injection, and by retroviral and adenoviral infection. Extensive coverage is also given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for both constitutive and conditional (Cre-lox or the Flp-frt systems) gene targeting. Additional chapters provide cutting-edge techniques for the cryopreservation of both male and female germlines, for the generation of transgenic sheep by nuclear transfer, and for the basic analysis of transgenic organisms.
Biomedical and Agricultural Applications of Animal Transgenesis Jim McWhir
Part II. Transgenesis in Invertebrate and Lower Vertebrate Species
Gene Transfer in Drosophila Mark J. O'Connor and William Chia
Part III. Transgenesis in the Mouse: Oocyte Injection
Oocyte Injection in the Mouse Gary A. J. Brown and Timothy J. Corbin
Part IV. Alternative Routes to the Germline
Adenoviral Infection Tohru Tsukui and Yutaka Toyoda
Retroviral Infection Richard A. Bowen
Part V. Transgenesis in the Mouse: The ES Cell Route
In Vitro Isolation of Murine Embryonic Stem Cells David Wells
Production of Chimeras Derived from Murine Embryonic Stem Cells David Wells
Gene Targeting Strategies David W. Melton
Cre/loxP Recombination System and Gene Targeting Ralf Kühn and Raul M. Torres
Part VI. Cryopreservation of Mouse Lines
Cryopreservation of Transgenic Mouse Lines Jillian M. Shaw and Naomi Nakagata
Ovarian Tissue Transplantation and Cryopreservation: Application to Maintenance and Recovery of Transgenic and Inbred Mouse Lines Jillian M. Shaw and A. O. Trounson
Part VII. Transgenesis in the Rat
Transgenesis in the Rat Linda J. Mullins, Gillian Brooker, and John J. Mullins
Part VIII. Transgenesis in Domestic Species
Generation of Transgenic Livestock by Pronuclear Injection A. John Clark
Transgenic Sheep from Cultured Cells Keith H. S. Campbell
Part IX. Characterization and Analysis of Transgenic Strains
Analysis of Transgenic Mice Stefan Selbert and Dominic Rannie
Biomedical and Agricultural Applications of Animal Transgenesis Jim McWhir
Part II. Transgenesis in Invertebrate and Lower Vertebrate Species
Gene Transfer in Drosophila Mark J. O'Connor and William Chia
Part III. Transgenesis in the Mouse: Oocyte Injection
Oocyte Injection in the Mouse Gary A. J. Brown and Timothy J. Corbin
Part IV. Alternative Routes to the Germline
Adenoviral Infection Tohru Tsukui and Yutaka Toyoda
Retroviral Infection Richard A. Bowen
Part V. Transgenesis in the Mouse: The ES Cell Route
In Vitro Isolation of Murine Embryonic Stem Cells David Wells
Production of Chimeras Derived from Murine Embryonic Stem Cells David Wells
Gene Targeting Strategies David W. Melton
Cre/loxP Recombination System and Gene Targeting Ralf Kühn and Raul M. Torres
Part VI. Cryopreservation of Mouse Lines
Cryopreservation of Transgenic Mouse Lines Jillian M. Shaw and Naomi Nakagata
Ovarian Tissue Transplantation and Cryopreservation: Application to Maintenance and Recovery of Transgenic and Inbred Mouse Lines Jillian M. Shaw and A. O. Trounson
Part VII. Transgenesis in the Rat
Transgenesis in the Rat Linda J. Mullins, Gillian Brooker, and John J. Mullins
Part VIII. Transgenesis in Domestic Species
Generation of Transgenic Livestock by Pronuclear Injection A. John Clark
Transgenic Sheep from Cultured Cells Keith H. S. Campbell
Part IX. Characterization and Analysis of Transgenic Strains
Analysis of Transgenic Mice Stefan Selbert and Dominic Rannie
Index."
Es gelten unsere Allgemeinen Geschäftsbedingungen: www.buecher.de/agb
Impressum
www.buecher.de ist ein Shop der buecher.de GmbH & Co. KG Bürgermeister-Wegele-Str. 12, 86167 Augsburg Amtsgericht Augsburg HRA 13309