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Single amino acid substitutions can lead to various diseases like Hypertrophic Cardiomyopathy dilated cardiomyopathy and other diseases. Hypertrophic Cardiomyopathy is an autosomal inherited disease in which the condition is being transmitted from one generation to another ,so far no proper treatment is there for these disease and is found to be about one in every five hundred persons, or likely half of the population of United States suffering from these disease. Mutations in that of Tropomyosin, Troponin has been found to be the cause of these disease; although these disease has shown to be have been linked with that of Myosin heavy chain and its binding protein, but we here are concerned with only Troponin and Tropomyosin, how these change might have occurred.