Two-Hybrid Screening
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High Quality Content by WIKIPEDIA articles! Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein?protein interactions and protein-DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively. The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD).…mehr

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High Quality Content by WIKIPEDIA articles! Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein?protein interactions and protein-DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively. The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD).