This is the first resource to fully cover the instrumentation, method development, and applications of Ultra-High Performance Liquid Chromatography (U-HPLC). It details both the benefits and limitations of this method in the pharmaceutical industry, clinical research, the food industry, and environmental services. It covers U-HPLC topics coupled with ultra-violet detector (UV) and mass spectrometer (MS), instrumentation frequently used in solving complex molecules. This is an essential reference for scientists who utilize chromatographic techniques, including in academia, as well as…mehr
This is the first resource to fully cover the instrumentation, method development, and applications of Ultra-High Performance Liquid Chromatography (U-HPLC). It details both the benefits and limitations of this method in the pharmaceutical industry, clinical research, the food industry, and environmental services. It covers U-HPLC topics coupled with ultra-violet detector (UV) and mass spectrometer (MS), instrumentation frequently used in solving complex molecules. This is an essential reference for scientists who utilize chromatographic techniques, including in academia, as well as pharmaceutical, biotechnology, chemical, food, environmental, and related industries.Hinweis: Dieser Artikel kann nur an eine deutsche Lieferadresse ausgeliefert werden.
QUANYUN ALAN XU, PhD, is a Senior Research Scientist in the Pharmacology and Analytical Laboratory Facility at the University of Texas MD Anderson Cancer Center. Dr. Xu is the coauthor of Analytical Methods for Therapeutic Drug Monitoring and Toxicology as well as more than seventy scientific papers and book chapters.
Inhaltsangabe
Preface vii Contributors ix 1. UHPLC Method Development 1 Shujun Chen and Alireza Kord 2. Method Transfer Between HPLC and UHPLC Platforms 31 Gregory K. Webster, Thomas F. Cullen, and Laila Kott 3. Practical Aspects of Ultrahigh Performance Liquid Chromatography 55 Naijun Wu, Christopher J. Welch, Theresa K. Natishan, Hong Gao, Tilak Chandrasekaran, and Li Zhang 4. Coupling UHPLC with MS: The Needs, Challenges, and Applications 95 Julie Schappler, Serge Rudaz, Jean-Luc Veuthey, and Davy Guillarme 5. The Potential of Shell Particles in Fast Liquid Chromatography 133 Szabolcs Fekete and Jeno Fekete 6. UHPLC Determination of Drugs of Abuse in Human Biological Matrices 169 Fabio Gosetti, Eleonora Mazzucco, and Maria Carla Gennaro 7. UHPLC in the Analyses of Isoflavones and Flavonoids 197 Sylwia Magiera and Irena Baranowska 8. UHPLC for Characterization of Protein Therapeutics 235 Jennifer C. Rea, Yajun Jennifer Wang, and Taylor Zhang 9. UHPLC/MS Analysis of Illicit Drugs 253 Guifeng Jiang, Jason R. Stenzel, Ray Chen, and Diab Elmashni 10. Ultra-High Performance Liquid Chromatography - Mass Spectrometry and Its Application 271 Zhili Xiong, Ying Deng, and Famei Li Index 291
Preface vii Contributors ix 1. UHPLC Method Development 1 Shujun Chen and Alireza Kord 2. Method Transfer Between HPLC and UHPLC Platforms 31 Gregory K. Webster, Thomas F. Cullen, and Laila Kott 3. Practical Aspects of Ultrahigh Performance Liquid Chromatography 55 Naijun Wu, Christopher J. Welch, Theresa K. Natishan, Hong Gao, Tilak Chandrasekaran, and Li Zhang 4. Coupling UHPLC with MS: The Needs, Challenges, and Applications 95 Julie Schappler, Serge Rudaz, Jean-Luc Veuthey, and Davy Guillarme 5. The Potential of Shell Particles in Fast Liquid Chromatography 133 Szabolcs Fekete and Jeno Fekete 6. UHPLC Determination of Drugs of Abuse in Human Biological Matrices 169 Fabio Gosetti, Eleonora Mazzucco, and Maria Carla Gennaro 7. UHPLC in the Analyses of Isoflavones and Flavonoids 197 Sylwia Magiera and Irena Baranowska 8. UHPLC for Characterization of Protein Therapeutics 235 Jennifer C. Rea, Yajun Jennifer Wang, and Taylor Zhang 9. UHPLC/MS Analysis of Illicit Drugs 253 Guifeng Jiang, Jason R. Stenzel, Ray Chen, and Diab Elmashni 10. Ultra-High Performance Liquid Chromatography - Mass Spectrometry and Its Application 271 Zhili Xiong, Ying Deng, and Famei Li Index 291
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