This study was conducted to establish the molecular systematics and historical biogeography of Genus Macrobrachium inferred from 16S rRNA. Specifically, this study was conducted to (1) reconstruct the phylogenetic tree of Genus Macrobrachium (2) infer the divergence time and evolutionary timescale of Genus Macrobrachium with two fossil calibrations, and (3) model the ancestral biogeographical history of Genus Macrobrachium from 16S rRNA gene sequences.
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