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A longitudinal study with a control group designed to evaluate the role of urine, serum proteins and the immunological response in the diagnosis ,prognosis ,follow up and treatment of breast cancer patients.ELIZA test of urine protein coating antigen provide a hope for the development of non-invasive breast cancer screening and or diagnostic laboratory test. Serum IgA, C3,IL-6,and TNF- are important prognostic indicator and could be a vital target in breast cancer therapy.CA15-3 is an important diagnostic, prognostic indicator and good predictor for disease relapse.