This study aims to analyze the factors that affect the use of natural resources for sustainable tourism development in the town of Pasaje canton, El Oro province, in 2016. The methodological research is of an explorative-deductive type with a quantitative approach applying research tools based on surveys with closed and objective questions, applied to 395 people with an admissible error of 5% in the result. Due to this, the results were verified and revealed that the relevant natural resources in the locality are rivers, medicinal and edible plants, which are of great contribution for the development not only of tourism but also of economic character. The present investigation showed that the canton of Pasaje has important natural resources, despite the lack of an inventory of these resources, Pasaje contributes to local tourism development in a sustainable manner.