This book enhances and provides you with basic idea of Jatropha Oil and its use as a biofuel in the Locomotives. Biofuels are the substitute for many fuels that are evolved. The Chapters discussed here gives you the detailed study of the Jatropha Oil, its Extraction Techniques,and the implementation of this oil with the typical diesel engine Kirloskar Engine. The obtained results are being compared with Diesel oil, methyl esters of jatropha oil. The Experimental Setup with the measurements after the practical solution are discussed with emissions of Smoke Intensity, Ho,Co,Nox,Exhaust Gas Temperature and thus obtained results are plotted with the graphs respectively. With this reference I conclude that Jatropha oil can also be used as a Biofuel in Various Engines and Locomotives.