Utilization of agro-industrial waste residues for amylase production - Fatima, Bilqees
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Six different agro-industrial waste residues (rice straw, rice bran, corn flakes, wheat bran, wheat flakes, and grinded wheat kernel) were procured from the local market. These substrates (10 g) were moistened (1:1) with different moistening agents (distilled water, tap water, mineral salts solution (FeSO4.7H2O 0.02, MgSO4.7H2O 1.0, (NH4)2SO4 4.0, KH2PO4 0.6, K2HPO4 1.4 mg/gds at pH 5), 0.1 N HCl, sodium acetate buffer (pH 5.5), sodium phosphate buffer pH 7.5) and screened for the production of 1,4- - D-glucan glucohydrolase for 96 hours in static cultures. The substrate and moistening agent…mehr

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Produktbeschreibung
Six different agro-industrial waste residues (rice straw, rice bran, corn flakes, wheat bran, wheat flakes, and grinded wheat kernel) were procured from the local market. These substrates (10 g) were moistened (1:1) with different moistening agents (distilled water, tap water, mineral salts solution (FeSO4.7H2O 0.02, MgSO4.7H2O 1.0, (NH4)2SO4 4.0, KH2PO4 0.6, K2HPO4 1.4 mg/gds at pH 5), 0.1 N HCl, sodium acetate buffer (pH 5.5), sodium phosphate buffer pH 7.5) and screened for the production of 1,4- - D-glucan glucohydrolase for 96 hours in static cultures. The substrate and moistening agent that gave maximum enzyme production were selected and their fermentation conditions were further optimized. The levels of selected solid substrate, moistening agent and fermentation conditions such as pH, temperature, time of incubation, inoculum size etc. were optimized by one variable at a time method. Aspergillus oryzae IIB-6 was found to be a good producer of 1,4- -D-glucan glucohydrolasein wheat bran medium containing mineral salts as an additional trace elements so that it can be used for biotechnological purposes.
Autorenporträt
Bilqees Fatima has a Bachelor of Science in Medical and Biological Sciences, A Master of Botany with a specialization in Fermentation Technology, a Master of Philosophy in Biotechnology/Biochemistry with a specialization in Protein Purification and a Ph.D in Biotechnology/Molecular Biology with a specialization in Genetic Engineering.