Vaccine Delivery Technology

Methods and Protocols
Herausgegeben:Pfeifer, Blaine A.; Hill, Andrew

• Gebundenes Buch

Produktbeschreibung

This volume discusses the vaccine development process and the role delivery concepts contribute to a global goal of effective health outcomes. The chapters in this book cover a wide range of topics such as antigen discovery methods; genetic and protein antigen preparation; preparation of viral vaccines as VLPs; viral and non-viral gene delivery; needle-less or non-invasive delivery technology; vaccine storage; and vaccine administration and assessment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics; lists of the necessary materials and reagents; step-by-step, readily reproducible laboratory protocols; and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Vaccine Delivery Technology: Methods and Protocols is a valuable resource for both novice and expert researchers, in and outside the field, who would like to gain insight into the impactful fieldof vaccines.
Chapter 7 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Produktdetails

• Methods in Molecular Biology 2183
• Verlag: Humana / Springer US / Springer, Berlin
• Artikelnr. des Verlages: 978-1-0716-0794-7
• 1st ed. 2021
• Seitenzahl: 608
• Erscheinungstermin: 22. September 2020
• Englisch
• Abmessung: 260mm x 183mm x 38mm
• Gewicht: 1295g
• ISBN-13: 9781071607947
• ISBN-10: 1071607944
• Artikelnr.: 58924734

Inhaltsangabe

Vaccine Delivery and Immune Response Basics.- Isolating Pathogen-Specific Human Monoclonal Antibodies (hmAbs) using Bacterial Whole Cells as Molecular Probes.- Use of Chlamydial Elementary Bodies as probes to Isolate Pathogen-Specific Human Monoclonal Antibodies.- Computational Antigen Discovery for Eukaryotic Pathogens using Vacceed.- Antigen Discovery in Bacterial Pan-Proteomes.- Alphavirus-Based Antigen Preparation.- Production of Chimeric Hepatitis B Virus Surface Antigens in Mammalian Cells.- Bioreactor-Based Antigen Production Process using the Baculovirus Expression Vector System.- High-Throughput Process Development for the Chromatographic Purification of Viral Antigens.- Production of Zika Virus Virus-Like Particles.- Biosynthesis of Glycoconjugate Virus-Like Particles (VLPs).- Up- and Downstream Processes for Viral Nanoplaxes as Vaccines.- Whole-Cell Vaccine Preparation: Options and Perspectives.- O-AntigenExtraction, Purification, and Chemical Conjugation to a Carrier Protein.- Oligosaccharide Antigen Conjugation to Carrier Proteins to Formulate Glycoconjugate Vaccines.- Exploitation of Capsule Polymerases for the Enzymatic Synthesis of the Polysaccharide Antigens used in Glycoconjugate Vaccines.- Attenuation Methods for Live Vaccines.- Surface Modification of Adenovirus Vector to Improve Immunogenicity and Tropism.- Production of Baculovirus and Stem Cells for Baculovirus-Mediated Gene Transfer into Human Mesenchymal Stem Cells.- Lipofection-Based Delivery of DNA Vaccines.- Flavivirus DNA Vaccine Design and Adjuvant Selection.- Vaccine Delivery with a Detoxified Bacterial Toxin.- Needle-Less or Non-Invasive Delivery Technology.- Lactic Acid Bacteria as Delivery Vehicle for Therapeutics Applications.- A Hybrid Biological-Biomaterial Vector for Antigen Delivery.- Liposomal Dual Delivery of Both Polysaccharide and Protein Antigens.- Thin-Film Freeze-Drying is a Viable Method to Convert Vaccines Containing Aluminum Salts from Liquid to Dry Powder.- The Application of Cryogenic Transmission Electron Microscopy for Evaluation of Vaccine Delivery Carriers.- Methods to Evaluate Immune Cell Recruitment and the Cellular Uptake and Distribution of Antigen following Intramuscular Administration of Vaccine to Mice.- The Quantification of Antigen-Specific T Cells by IFN-Gamma ELISpot.- The Measurement of Antigen-Specific IgG Titers by Direct ELISA.- Method to Evaluate In Vivo CD8+ T Cell Cytotoxicity in a Murine Model.- Testing Anti-Pneumococcal Antibody Function using Bacteria and Primary Neutrophils.- Viral Infectivity Qunatification and Neutralization Assays using Laser Force Cytology.