This volume provides a practical guide providing step-by-step protocol to design and develop vaccines for human diseases. Divided into three volumes, Volume 1: Vaccines for Human Diseases guides readers through an introductory section on future challenges for vaccinologists and the immunological mechanism of vaccines. Chapters focus on design of human vaccines for viral, bacterial, fungal, and parasitic diseases as well as tumor vaccines. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary…mehr
This volume provides a practical guide providing step-by-step protocol to design and develop vaccines for human diseases. Divided into three volumes, Volume 1: Vaccines for Human Diseases guides readers through an introductory section on future challenges for vaccinologists and the immunological mechanism of vaccines. Chapters focus on design of human vaccines for viral, bacterial, fungal, and parasitic diseases as well as tumor vaccines. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and practical, Vaccine Design: Methods and Protocols, Second Edition, Volume 1: Vaccines for Human Diseases aims to be a useful practical guide to researchers to help further their study in this field.
Challenges for Vaccinologists in the First Half of the Twenty First Century.- Principles in Immunology for the Design and Development of Vaccines.- Revisiting the Principles of Designing a Vaccine.- Status of COVID-19 Pandemic before the Administration of Vaccine.- mRNA Vaccines to Protect against Diseases.- Artificial Intelligence in Vaccine and Drug Design.- Vaccines Targeting Numerous Coronavirus Antigens, Ensuring Broader Global Population Coverage: Multi-Epitope and Multi-Patch Vaccines.-Use of Micro-computed Tomography to Visualize and Quantify COVID-19 Vaccine Efficiency in Free-breathing Hamsters.- Design of Replication-Competent VSV- and Ervebo-vectored Vaccines against SARS-CoV-2.- CRISPR Engineering of Bacteriophage T4 to Design Vaccines against SARS-CoV-2 and Emerging Pathogens.- Techniques for Developing and Assessing Immune Responses Induced by Synthetic DNA Vaccines for Emerging Infectious Diseases.- Towards Determining the Epitopes of the Structural proteins of SARS-CoV-2.- Development, production and characterization of hepatitis B subviral envelope particles as a third-generation vaccine.- Generation of CpG-Recoded Zika Virus Vaccine Candidates.- Salmonella Uptake into Gut-Associated Lymphoid Tissues: Implications for Targeted Mucosal Vaccine Design and Delivery.- Development of Human Recombinant Leptospirosis Vaccines.- Induction of T-cell Responses by Vaccination of a Streptococcus pneumoniae Whole Cell Vaccine.- Development of a Bacterial Nanoparticle Vaccine against Escherichia coli.- Construction of Novel Live Genetically Modified BCG Vaccine Candidates using Recombineering Tools.- An Update on Tuberculosis Vaccines.- Structure-Based Design of Diagnostics and Vaccines for Lyme Disease.- Development of a SONIX vaccine to protect against ehrlichiosis.- Development of the Anti-Leishmanial Vaccine.- In Silico Design of Recombinant ChimeraT Cell Peptide Epitope Vaccines for Visceral Leishmaniasis.- Pre-clinical Assessment of the Immunogenicity of Experimental Leishmania Vaccines.- Production of Oral Vaccines Based on Retrovirus Virus-like Particles Pseudotyped with Protozoan-Surface Proteins.- A Fast-Track Phenotypic Characterization of Plasmodium falciparum Vaccine Antigens through Lyse-Reseal Erythrocytes Mediated Delivery (LyRED) of RNA Interference for Targeted Translational Repression.- Plasmodium falciparum Antigen Expression in Leishmania Parasite: A Way Forward for Live Attenuated Vaccine Development.- Molecular Characterization of a Vector-based Candidate Antigen using the 3'-RACE and Genome Walking Methods and In Silico Analysis of the Correspondent Protein for Vaccine Design and Development.- In vitro culture of Plasmodium falciparum for the Production of Mature Gametocytes for Performing Standard Membrane Feeding Assay and Infection of Anopheles spp..- Plasmodium berghei Infection in BALB/c Mice Model as an Animal Model for Malaria Disease Research.- Standard membrane feeding assay for malaria transmission studies.- Generation of Tumor Targeted Dendritic Cell Vaccines with Improved Immunogenic and Migratory Phenotype.- Monocytes as a Cellular Vaccine Platform to Induce Anti-Tumor Immunity.- Beyond Sequencing: Prioritizing and Delivering Neoantigens for Cancer Vaccines.- Proteomics for Development of Food Allergy Vaccines.- Estimating Vaccine Potency using Antibody-based Competition Assays.
Challenges for Vaccinologists in the First Half of the Twenty First Century.- Principles in Immunology for the Design and Development of Vaccines.- Revisiting the Principles of Designing a Vaccine.- Status of COVID-19 Pandemic before the Administration of Vaccine.- mRNA Vaccines to Protect against Diseases.- Artificial Intelligence in Vaccine and Drug Design.- Vaccines Targeting Numerous Coronavirus Antigens, Ensuring Broader Global Population Coverage: Multi-Epitope and Multi-Patch Vaccines.-Use of Micro-computed Tomography to Visualize and Quantify COVID-19 Vaccine Efficiency in Free-breathing Hamsters.- Design of Replication-Competent VSV- and Ervebo-vectored Vaccines against SARS-CoV-2.- CRISPR Engineering of Bacteriophage T4 to Design Vaccines against SARS-CoV-2 and Emerging Pathogens.- Techniques for Developing and Assessing Immune Responses Induced by Synthetic DNA Vaccines for Emerging Infectious Diseases.- Towards Determining the Epitopes of the Structural proteins of SARS-CoV-2.- Development, production and characterization of hepatitis B subviral envelope particles as a third-generation vaccine.- Generation of CpG-Recoded Zika Virus Vaccine Candidates.- Salmonella Uptake into Gut-Associated Lymphoid Tissues: Implications for Targeted Mucosal Vaccine Design and Delivery.- Development of Human Recombinant Leptospirosis Vaccines.- Induction of T-cell Responses by Vaccination of a Streptococcus pneumoniae Whole Cell Vaccine.- Development of a Bacterial Nanoparticle Vaccine against Escherichia coli.- Construction of Novel Live Genetically Modified BCG Vaccine Candidates using Recombineering Tools.- An Update on Tuberculosis Vaccines.- Structure-Based Design of Diagnostics and Vaccines for Lyme Disease.- Development of a SONIX vaccine to protect against ehrlichiosis.- Development of the Anti-Leishmanial Vaccine.- In Silico Design of Recombinant ChimeraT Cell Peptide Epitope Vaccines for Visceral Leishmaniasis.- Pre-clinical Assessment of the Immunogenicity of Experimental Leishmania Vaccines.- Production of Oral Vaccines Based on Retrovirus Virus-like Particles Pseudotyped with Protozoan-Surface Proteins.- A Fast-Track Phenotypic Characterization of Plasmodium falciparum Vaccine Antigens through Lyse-Reseal Erythrocytes Mediated Delivery (LyRED) of RNA Interference for Targeted Translational Repression.- Plasmodium falciparum Antigen Expression in Leishmania Parasite: A Way Forward for Live Attenuated Vaccine Development.- Molecular Characterization of a Vector-based Candidate Antigen using the 3'-RACE and Genome Walking Methods and In Silico Analysis of the Correspondent Protein for Vaccine Design and Development.- In vitro culture of Plasmodium falciparum for the Production of Mature Gametocytes for Performing Standard Membrane Feeding Assay and Infection of Anopheles spp..- Plasmodium berghei Infection in BALB/c Mice Model as an Animal Model for Malaria Disease Research.- Standard membrane feeding assay for malaria transmission studies.- Generation of Tumor Targeted Dendritic Cell Vaccines with Improved Immunogenic and Migratory Phenotype.- Monocytes as a Cellular Vaccine Platform to Induce Anti-Tumor Immunity.- Beyond Sequencing: Prioritizing and Delivering Neoantigens for Cancer Vaccines.- Proteomics for Development of Food Allergy Vaccines.- Estimating Vaccine Potency using Antibody-based Competition Assays.
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