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High Quality Content by WIKIPEDIA articles! Valentine National Wildlife Refuge is located in the U.S. state of Nebraska and includes 19,131 acres (77.42 km²). The refuge protects a portion of the largest remaining area of tall and mid grass prairie remaining in the U.S. Collectively known as the sand hills region of Nebraska, the dunes were the end result of the last ice age known as the Pinedale glaciation. During the Holocene glacial retreat the sand dunes that been deposited in their current location by the vast continental glaciers, were exposed and grasses eventually took over. The refuge is managed by the U.S. Fish and Wildlife Service and along with Fort Niobrara and John and Louise Seier National Wildlife Refuges, they form the Fort Niobrara National Wildlife Refuge Complex.