Our study has shown the effect of climatic and soil factors on the chemical composition and biological activities of the medicinal plant Mentha spicata.L. We were chosen from three different regions, El Oued located in the south and characterized by Saharan climate, El Tarf located in the far northeast and characterized by temperate climate, Tébessa located in the northeast and characterized by semi-arid climate. Activities The biological properties of Mentha spicata.L vary depending on the plant part used, the climatic and soil conditions of the plant's growing areas. The results obtained were shown that Mentha spicata.L growing in desert areas with sandy soil contains a higher yield of phenolic compounds. It was also noted from the results obtained that Mentha spicata harvested from El Oued presents the better antioxidant, anti-hemolytic and anti-diabetic activity and Mentha spicata harvested from El Tarf presents better analgesic activity. The antimicrobial activity is different depending on the microbial strains and regions studied.