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Onion peel act as a good source for the growth of Penicillium chrysogenum than Aspergillus niger. It is very rare to see the single fungal culture in one plate without any other fungal contamination and the rare case was observed in our research in Onion peel medium with Penicillium chrysogenum. Formulation of Selective medium for fungi is very challenging in the present scenario. This present research will provide a way for the production of low cost Selective medium for Penicillium chrysogenum and other fungal isolates. Garlic peel showed an average growth for Penicillium chrysogenum and the fungi Aspergillus niger did not showed any growth on Garlic peel medium. Finally, in our research we concluded that the Onion peel waste was the good low cost environmental free medium for the cultivation of an industrially important fungi Penicillium chrysogenum and Aspergillus niger. Garlic peel waste medium is the good source for Penicillium chrysogenum cultivation but not for the cultivation of Aspergillus niger. The present study will provide the concept of "Zero waste economy" to this society.