Video microscopy is used extensively in many life and biomedical science disciplines today, and is a useful tool for both cell biologists and students. This book presents how to track the dynamic changes that take place in the structure of living cells and in reconstituted preparations using video and digital imaging microscopy. Basic information, principles, and applications are also covered, as well as more specialized video microscopy techniques. Key Features* Chapters cover the commonly used video technologies in biological research* Nontechnical presentation of principles* Emphasis on the…mehr
Video microscopy is used extensively in many life and biomedical science disciplines today, and is a useful tool for both cell biologists and students. This book presents how to track the dynamic changes that take place in the structure of living cells and in reconstituted preparations using video and digital imaging microscopy. Basic information, principles, and applications are also covered, as well as more specialized video microscopy techniques. Key Features* Chapters cover the commonly used video technologies in biological research* Nontechnical presentation of principles* Emphasis on the practical aspects of instrument use* Covers pitfalls in instrument uses that can lead to artifacts* Authors are leaders in the design and application of video methods to biological microscopy* Presentation of material tailored for the established researcher that has little experience with video methods* Examples are extensively illustrated with photographs
G. Sluder and E.H. Hinchcliffe Video Basics: Use of Camera and Monitor Adjustments. K.A. Jacobsen and K. Berland Electronic Cameras for Low Light Microscopy. M. Oshiro Cooled CCD vs Intensified Cameras forLow Light Video--Applications and Relative Advantages. T. Inoue and N. Gliksman Techniques for Optimizing Microscopy and Analysis Through Digital Image Processing. R. Cardullo Basics of Image Processing: Analog and Digital. D. Wolf Quantitative Video Microscopy. E. Keller Proper Alignment of the Microscope. J. Hinsch Mating Cameras to Microscopes. E.D. Salmon and P. Tran High Resolution Video-Enhanced Differential Interference Contrast (VE-DIC) Light Microscopy. E.D. Salmon S.L. Shaw J. Waters C.M. Waterman-Storer P.S. Maddox E. Yeh and K. Bloom A High-Resolution Multi-Mode Digital Microscope System. F.R. Maxfield and K. Dunn Ratio Imaging Instrumentation. R.B. Silver Ratio Imaging: Practical Considerations for Measuring Intracellular Calcium and pH in Living Tissue. C. Rieder and R.W. Cole Perfusion Chambers for High Resolution Video Light Microscopic Studies of Vertebrate Cell Monolayers: Some Considerations and a Design. E. Gratton P.T.C. So C.Y. Dong T. French and K. Berland Fluorescence Lifetime Imaging Techniques for Microscopy. Y.-L. Wang Digital Deconvolution of Fluorescence Images for Biologists. Subject Index.
G. Sluder and E.H. Hinchcliffe Video Basics: Use of Camera and Monitor Adjustments. K.A. Jacobsen and K. Berland Electronic Cameras for Low Light Microscopy. M. Oshiro Cooled CCD vs Intensified Cameras forLow Light Video--Applications and Relative Advantages. T. Inoue and N. Gliksman Techniques for Optimizing Microscopy and Analysis Through Digital Image Processing. R. Cardullo Basics of Image Processing: Analog and Digital. D. Wolf Quantitative Video Microscopy. E. Keller Proper Alignment of the Microscope. J. Hinsch Mating Cameras to Microscopes. E.D. Salmon and P. Tran High Resolution Video-Enhanced Differential Interference Contrast (VE-DIC) Light Microscopy. E.D. Salmon S.L. Shaw J. Waters C.M. Waterman-Storer P.S. Maddox E. Yeh and K. Bloom A High-Resolution Multi-Mode Digital Microscope System. F.R. Maxfield and K. Dunn Ratio Imaging Instrumentation. R.B. Silver Ratio Imaging: Practical Considerations for Measuring Intracellular Calcium and pH in Living Tissue. C. Rieder and R.W. Cole Perfusion Chambers for High Resolution Video Light Microscopic Studies of Vertebrate Cell Monolayers: Some Considerations and a Design. E. Gratton P.T.C. So C.Y. Dong T. French and K. Berland Fluorescence Lifetime Imaging Techniques for Microscopy. Y.-L. Wang Digital Deconvolution of Fluorescence Images for Biologists. Subject Index.
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