Vincenzo Anastagi (died 1585) was an Italian Knight of Rhodes who participated in the Siege of Malta (1565). He had joined the Knights two years before the siege. Sallying with about a hundred cavalry from Mdina, he led a surprise cavalry charge against the poorly guarded Turkish camp on August 7th, while the main Turkish force was engaged in an assault on Birgu and Senglea. The assault would have likely succeeded were it not for the intervention of Anastagi's cavalry that created a confusion in the Turkish rear.