Wood Formation in Trees

Cell and Molecular Biology Techniques
Herausgeber: Chaffey, Nigel J

• Broschiertes Buch

Produktbeschreibung

Trees, a major component of the biosphere, can provide solutions to modern challenges of global warming and dwindling fossil fuel reserves. Unfortunately, too little is known about the biology of these plants, due to a lack of appropriate techniques for studying how they are formed. Wood Formation in Trees presents a variety of detailed protocols for the study of the cell and molecular biology of wood formation: anatomical, biochemical and molecular-genetic. Internationally recognized experts, most of whom are the researchers who developed the techniques, speak with authority in this volume, and also provide first-hand tips and trade secrets to help the uninitiated master the techniques.

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Produktdetails

• Verlag: CRC Press
• Seitenzahl: 384
• Erscheinungstermin: 29. Oktober 2019
• Englisch
• Abmessung: 277mm x 208mm x 20mm
• Gewicht: 1134g
• ISBN-13: 9780367396404
• ISBN-10: 0367396408
• Artikelnr.: 58054264

Autorenporträt

Nigel Chaffey

Inhaltsangabe

Introduction. An Introduction to the Problems of Working with Trees. Wood
Microscopical Techniques. Conventional (Chemical-fixation) Transmission
Electron Microscopy and Cytochemistry of Angiosperm Trees. Chemical and
Cryo-fixation for Transmission Electron Microscopy of Gymnosperm Cambial
Cells. Deep-etching Electron Microscopy and 3-Dimensional Cell Wall
Architecture. Secondary Ion Mass Spectometry Microscopy: Application to the
Analysis of Woody Samples. Immunolocalization of the Cytoskeleton in the
Secondary Vascular System of Angiosperm Trees and its Visualisation Using
Epifluorescence Microscopy. Immunolocalization and Visualization of the
Cytoskeleton in Gymnosperms using Confocal Laser Scanning Microscopy. Cell
Walls of Woody Plants: Autoradiography and Ultraviolet Microscopy. Cell
Walls of Woody Tissues: Cytochemical, Biochemical and Molecular Analysis of
Pectins and Pectin Methylesterases. Immunolocalization of Enzymes of
Lignification. Sampling of Cambial Region Tissues for High Resolution
Analysis. Biochemistry and Quantitative Histochemistry of Wood. Protein
Analysis in Perennial Tissues. The Use of GUS Histochemistry to Visualize
Lignification Gene Expression In Situ during Wood Formation. In Situ
Hybridization. Random Amplification of Polymorphic DNA and Reverse
transcription Chain Reaction of RNA in Studies of Sapwood and Heartwood.