This monograph deals with zonate leaf spot of sorghum which is one of the most destructive diseases of the crop caused by a fungal pathogen Gloeocercospora sorghi. Several basic techniques alongwith their experimental results, have been discussed in very detail, simple and lucid manner. The techniques include: 1. Isolation, Purification and Preservation of fungus from diseased sample, 2. In vitro screening of biocontrol agents (Dual culture technique) and fungicides (Poisoned food technique) and 3.Seed biopriming.