The painting underwent rigorous forensic examination, combining historical research with both invasive and non-invasive scientific imaging techniques. The analysis utilized advanced physical and chemical instrumentation to determine the painting's authenticity and accurate chronological placement.
A comparative review of published chemical analyses of pigments, dyes, and substrates used in Raphael's works from collections worldwide is included. Additionally, this study explores the innovative use of artificial intelligence (AI) for facial comparison between the figures in the tondo painting, the Sistine Madonna, and other Raphael artworks. These AI-generated insights provide novel information about the identities of Raphael's models and shed light on his working techniques, as well as those of his associates.
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