Adenovirus Methods and Protocols (eBook, PDF)

Volume 1: Adenoviruses, Ad Vectors, Quantitation, and Animal Models
Redaktion: Wold, William S. M.; Tollefson, Ann E.

• Format: PDF

Produktbeschreibung

Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding the first edition, the authors have added new chapters that address innovative areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. Each of the protocols presented in these volumes is written by trendsetting researchers.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 242
• Erscheinungstermin: 2. Februar 2008
• Englisch
• ISBN-13: 9781597451666
• Artikelnr.: 64700582

Autorenporträt

William S. M. Wold, St. Louis University, St. Louis, MO, USA / Ann E. Tollefson, St. Louis University, St. Louis, MO, USA

Inhaltsangabe

Manipulation of Early Region 4.- Isolation, Growth, and Purification of Defective Adenovirus Deletion Mutants.- Construction of Adenovirus Type 5 Early Region 1 and 4 Virus Mutants.- Construction of Mouse Adenovirus Type 1 Mutants.- Generation of Recombinant Adenovirus Using the Escherichia coli BJ5183 Recombination System.- Production and Release Testing of Ovine Atadenovirus Vectors.- Construction of Capsid-Modified Recombinant Bovine Adenovirus Type 3.- Adenovirus Capsid Chimeras.- Temperature-Sensitive Replication-Competent Adenovirus shRNA Vectors to Study Cellular Genes in Virus-Induced Apoptosis.- Evaluating Apoptosis in Tumor Cells Infected With Adenovirus Expressing p53 Tumor Suppressor Gene.- Growth and Purification of Enteric Adenovirus Type 40.- Immunocompetent, Semi-Permissive Cotton Rat Tumor Model for the Evaluation of Oncolytic Adenoviruses.- Use of the Syrian Hamster as an Animal Model for Oncolytic Adenovirus Vectors.- A Real-Time PCR Method to Rapidly Titer Adenovirus Stocks.- Detection and Quantitation of Subgroup C Adenovirus DNA in Human Tissue Samples by Real-Time PCR.- Flow Cytometric Detection of Adenoviruses and Intracellular Adenovirus Proteins.- Capture ELISA Quantitation of Mouse Adenovirus Type 1 in Infected Organs.- Preparation and Titration of CsCl-Banded Adenovirus Stocks.

Rezensionen

"...provdes some good advice on using and growing adenovirus vectors." -Doody's Book Review, Weighted Numerical Score: 62 - 2 Stars

Reviews of the First edition:

"This book caters quite well to those who simply want to grow adenoviruses, assay them, and use them. . .a very comprehensive methods book that will be a laboratory standard for years to come."-Microbiology Today

". . .this book constitutes an excellent reference text . . .this book is replete with useful information for both the virologist and the non-virologist who is interested in working with adenoviruses."-FEBS Letters