Adrenergic Receptor Protocols (eBook, PDF)

Redaktion: Machida, Curtis A.

• Format: PDF

Produktbeschreibung

In Adrenegic Receptor Protocols, Curtis Machida and a panel of expert investigators present a comprehensive collection of modern molecular methods for analyzing adrenergic receptors and corresponding second messenger systems. These proven and readily reproducible techniques utilize genetic, RNA, protein expression, transactivator, and second messenger methodologies, as well as immunocytochemical, electrophysiological, transgenic, and in situ hybridization approaches. Each of the experts writing here details the use of their chosen method in examining the adrenergic receptor system, using aspects of the genetic flow of information as a guide: DNA ' RNA ' transactivator ' protein expression ' second messenger analyses ' cellular analyses ' transgenic whole animal approaches. Comprehensive and rich in practical detail, Adrenergic Receptor Protocols provides the first collection of reproducible methods for the study of these important regulators of CNS-mediated behavior and neural function. Its state-of-the-art methods constitute today's gold standard reference for all neurobiologists, neurochemists, neurologists, and pharmacologists studying this exceptionally important class of receptors.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 577
• Erscheinungstermin: 3. Februar 2008
• Englisch
• ISBN-13: 9781592596843
• Artikelnr.: 64700771

Inhaltsangabe

Genetic Analysis.- Construction of Libraries for Isolation of Adrenergic Receptor Genes.- Isolation of Adrenergic Receptor Genes.- Analyses of Adrenergic Receptor Sequences.- Polymerase Chain Reaction Screening of Genomic Libraries for Adrenergic Receptor Genes.- Solution-Phase Library Screening for Identification of Rare Adrenergic Receptor Clones.- Genetic Polymorphisms of Adrenergic Receptors.- RNA Analysis.- Northern Blot Analyses Detecting Adrenergic Receptor mRNAs.- Ribonuclease Protection Assay for the Detection of ?1-Adrenergic Receptor RNA.- Determination of Adrenergic Receptor mRNAs by Quantitative Reverse Transcriptase-Polymerase Chain Reactions.- Nuclear Run-On Assays for Measurement of Adrenergic Receptor Transcription Rate.- Primer Extension Methods for Determination of ?1-Adrenergic Receptor mRNA Start Sites.- Expression Analysis.- Use of Eukaryotic Vectors for the Expression of Adrenergic Receptors.- Expression of ?-Adrenergic Receptors in Recombinant Baculovirus-Infected Insect Cells.- Expression of ?-Adrenergic Receptors in E. coli.- Use and Pharmacological Analysis of Established and Transfected Cell Lines Expressing Adrenergic Receptors.- Transient Transfection and Adrenergic Receptor Promoter Analysis.- Antisense RNA/DNA-Based Techniques to Probe Adrenergic Receptor Function.- Targeted Disruption of Adrenergic Receptor Genes.- Receptor and Second Messenger Analysis.- Development of Antibodies to Adrenergic Receptors.- Western Blot Detection of Adrenergic Receptors.- UV Crosslinking of Adrenergic Receptors and Ligands.- Detection of ?-Adrenergic Receptors by Radioligand Binding.- Assessing Adrenergic Receptor Conformation Using Chemically Reactive Fluorescent Probes.- Biochemical Methods for Detection and Measurement of Cyclic AMP and AdenylylCyclase Activity.- Assay of Arachidonic Acid Release Coupled to ?1- and ?2-Adrenergic Receptors.- Patch-Clamp Recording Methods for Examining Adrenergic Regulation of Potassium Currents in Ocular Epithelial Cells.- Transactivator Analysis.- Southwestern Blots for Detection of a DNA Binding Protein Recognizing the ?1B-Adrenergic Receptor Gene Promoter.- DNase I Footprinting Analysis of Transcription Factors Recognizing Adrenergic Receptor Gene Promoter Sequences.- Electrophoretic Mobility Shift Assay for Detection of DNA Binding Proteins Recognizing ?-Adrenergic Receptor Gene Sequences.- Determination of mRNA Stability and Characterization of Proteins Interacting with Adrenergic Receptor mRNAs.- Microscopy Analysis.- Use of Immunohistochemistry and Confocal Microscopy in the Detection of Adrenergic Receptors.- Distribution of ?2A- and ?2C-Adrenergic Receptor Immunoreactivity in the Central Nervous System.- Quantitative Light Microscopic Autoradiography of ?2-Adrenergic Radioligand Binding Sites.- In Situ Hybridization of Adrenergic Receptor mRNA in Brain.- Use of Electron Microscopy in the Detection of Adrenergic Receptors.