Advanced Techniques in Soil Microbiology (eBook, PDF)

Redaktion: Varma, Ajit; Oelmüller, Ralf

• Format: PDF

Produktbeschreibung

This book presents a wide range of biotechnological methods for application in soil microbiology analysis, including all essential methods involving molecular biology, immunology, microbiology, and structural biology, such as transcriptome analysis, RNAi technology, molecular matchmaking, RAPD, T-RFLP and FT/MS. The techniques and procedures presented here offer practical guides for immediate use in the laboratory. This volume will be of use both to the first-timer and to the experienced scientist.

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Produktdetails

• Verlag: Springer Berlin Heidelberg
• Seitenzahl: 427
• Erscheinungstermin: 25. Juli 2007
• Englisch
• ISBN-13: 9783540708650
• Artikelnr.: 37371010

Autorenporträt

Ajit Varma, Amity Institute, Noida, India / Ralf Oelmüller, University of Jena, Germany

Inhaltsangabe

Detection and Diversity of Fungi from Environmental Samples: Traditional Versus Molecular Approaches.- Functional Genomic Approaches for Mycorrhizal Research.- Automated Fluoroscence Sequencing and Troubleshooting.- mRNA Quantitation Using Real Time PCR.- Laboratory Practice for the Production of Polyclonal and Monoclonal Antibodies.- Modern Techniques for Analyzing Immunological Responses.- Transcriptome Analysis.- RNAi Technology: a Tool for Functional Validation of Novel Genes.- Molecular Matchmaking: Techniques for Biomolecular Interactions.- Environmental Proteomics: Extraction and Identification of Protein in Soil.- DGGE and RISA Protocols for Microbial Community Analysis in Soil.- Soil Microbial Community Structure and Function Assessed by FAME, PLFA and DGGE - Advantages and Limitations.- Measurement of Microbial Biomass and Activity in Soil.- Immuno-Technology for the Localization of Acid Phosphatase Using Native Gel Bands in Piriformospora indica and Other Soil Microorganisms.- Use of Short Oligonucleotide Primers in Random Amplified Polymorphic DNA Techniques for Species Identification.- Co-Cultivation with Sebacinales.- Quantitative Histochemistry: a Forgotten Tool with New Applications.- Ion Cyclotron Resonance Fourier Transform Mass Spectrometry for Non-Targeted Metabolomics of Molecular Interactions in the Rhizosphere.- Application of Terminal-Restriction Fragment Length Polymorphism for MolecularAnalysis of Soil Bacterial Communities.- Molecular Symbiotic Analysis Between Arabiopsis thaliana and Piriformospora indica.- Biophysical Phenomics Reveals Functional Building Blocks of Plants Systems Biology: a Case Study for the Evaluation of the Impact of Mycorrhization with Piriformospora indica.- Analysis of the Plant Protective Potential of the RootEndophytic Fungus Piriformospora indica in Cereals.- Members of Sebacinales Confer Resistance Against Heavy Metal Stress in Plants.- Screening of Plant Growth-Promoting Rhizobacteria.- Research Methods in Arbuscular Mycorrhizal Fungi.- Field Trials of Bioinoculants.

Rezensionen

Aus den Rezensionen:

"... Advanced Techniques in Soil Microbiology wendet sich an den Praktiker, der aktuelle biochemische Methoden direkt im Labor anwenden möchte. Dieses Ziel wird dadurch erreicht, dass die Methoden in Listenform Schritt für Schritt erklärt werden. Besonders hervorgehoben werden sollte hierbei, dass auch kleinere Tipps ... in dem Buch ... eine Erwähnung finden. ... Diese ... Tipps werden ... kurz erklärt, wodurch das Studium der Protokolle auch für den erfahrenen ... Wissenschaftler durchaus Neues zu bieten hat ..." (Jost Liebich, in: GDCh Mitteilungsblatt Gesellschaft Deutscher Chemiker, 2008, Issue 1, S. 17 f.)