Analytical Ultracentrifugation (eBook, PDF)

Techniques and Methods
Redaktion: Scott, David; Rowe, Arther; Harding, Stephen E

• Format: PDF

Produktbeschreibung

Analytical ultracentrifugation has become an increasingly important technique for monitoring the size and shape of biological macromolecules. Analytical Ultracentrifugation: Techniques and Methods contains contributions from experts in the field, bringing together the multitude of developments that have taken place in instrumentation and analysis over the past decade into a single volume. This book covers the latest methods in analysis along with an extensive introduction for the novice user. Analysis methods in both sedimentation velocity and sedimentation equilibrium are discussed at length. Protein, protein/DNA, membrane proteins and polymer systems are also explored, along with software developments and non-ideality.

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Produktdetails

• Verlag: RSC
• Seitenzahl: 586
• Erscheinungstermin: 16. Dezember 2007
• Englisch
• ISBN-13: 9781847552617
• Artikelnr.: 44762233

Inhaltsangabe

Contents and front matter; A Brief Introduction to the Analytical
Ultracentrifugation of Proteins for Beginners; Diffusion-Deconvoluted
Sedimentation Coefficient Distributions for the Analysis of Interacting and
Non-Interacting Protein Mixtures; Models for Direct Boundary Fitting of
Indefinite Ligand-Linked Self-Association; Allowance for Thermodynamic
Nonideality in Sedimentation Equilibrium; Using Analytical
Ultracentrifugation of DNA in CsCl Gradients to Explore Large-Scale
Properties of Genomes; LAMMNUM: A Program to StudySelf-Associating
Macromolecules in Sedimentation Velocity Experiments; "Correcting for the
Buoyancy of Macromolecules: Density Increments and Apparent Partial
Specific Volumes with Particular Reference to the Study of Membrane
Proteins;" Application of Weighted Robust Regression to Equilibrium
Ultracentrifugation; The Synthetic Boundary Technique of Analytical
Ultracentrifugation: A Valuable Tool to Study Structure Formation Processes
Online; Protein-DNA Interactions Studied at Sedimentation Equilibrium;
UltraScan - A Comprehensive Data Analysis Software Package for Analytical
Ultracentrifugation Experiments; Analysis of Polysaccharide Size, Shape and
Interactions; Analytical Ultracentrifugation as a Key Complementary
Technique in Structural Biology; Introduction to Differential
Sedimentation; Relating Small Angle Scattering and Analytical
Ultracentrifugation in Multidomain Proteins; Modelling of Biopolymers;
Fitting of Thermodynamic Data from Equilibrium Ultracentrifugation by
Robust Least-Squares with Reduced Parameter Cross-Correlation Coefficients;
Calculation of Volume, Surface, and Hydration Properties of Biopolymers;
Analysis of Membrane Proteins Using Analytical Ultracentrifugation;
Solution Properties of Flexible Macromolecules: Theoretical and
Computational Approaches; The ELLIPS Suite of Whole-Body Protein
Conformation Algorithms for Microsoft WINDOWS; Weak Interactions: Optimal
Algorithms for Their Study in the AUC; Analytical Ultracentrifugation of
Colloids; Index